KRAS Knockout cell line (Hep G2)

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KRAS Knockout cell line (Hep G2) Order

KRAS Knockout cell line (Hep G2)
Catalog#
YKO-HT5476
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
KRAS Knockout cell line (Hep G2)
Order

Detailed Product Information

Product category
KO Cell Lines
Cell name
KRAS Knockout cell line (Hep G2)
Species
Human
Gene Name
KRAS
Gene ID
3845
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 3 (based on transcript KRAS-202)
Validation: PCR+Sanger Sequencing

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the KRAS gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the Hep G2 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, KRAS gene knockout KRAS Knockout cell line (Hep G2) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
KRAS Knockout cell line (Hep G2)
KRAS Knockout cell line (Hep G2)
Catalog#
YKO-HT5476
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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